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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?
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Web streams do provide clear mechanisms for tuning backpressure behavior in the form of the highWaterMark option and customizable size calculations, but these are just as easy to ignore as desiredSize, and many applications simply fail to pay attention to them.
"He's going to have to prove himself a huge amount."